Composite

Part:BBa_K137078:Design

Designed by: Doug Tischer   Group: iGEM08_Caltech   (2008-08-19)


AHL -> H2O2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4031
    Illegal BglII site found at 4966
    Illegal BglII site found at 5093
    Illegal BamHI site found at 2741
    Illegal BamHI site found at 4883
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1005


Design Notes

None.


Source

Genomic PCR for the pyruvate oxidase (from Streptococcus pnumoniae R6) and catalase (katG from E. coli DH10B), while the rest of the construct is made out of parts from the registry.

References